We would like to show you a description here but the site won’t allow us. Find regional support. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 6% for the on-board optics (monochromators). Category 2 and 3 microplate readers use gradient heating as well. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Reporter Assay. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Capture Flash Assays with Ease. Figure 2-23: Bottle holder To install the bottle holder: 1. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax i2X is used for cell migration, cell. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. 04, 0. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. . applications, including dual luciferase and ATP assays. Find regional support. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. Increased fluorescence performance. Contact our support team with your questions regarding Molecular Devices products and applications. Patented user-exchangeable cartridge design expands the system's detection capability making it. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Art-science interactive light show/animation. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. The dynamic range of the assay is about 3. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. com. Use the 3. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. no. 1) Select the correct plate and the wells that you want to measure. , 2021). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The chamber. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 価格、見積もり、お近くの販売店を. The SpectraMax i3x from Molecular Devices is a multi-mode plate reader that can measure spectral-based absorbence and luminescence. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. This way you’ll save time on finding the necessary info. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. applications, including dual luciferase and ATP assays. *On the SpectraMax i3x reader only. SpectraMax i3x Multi-Mode Detection Platform. Description 1. Methods. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. These concentrations are then used to perform western blots. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Molecular Devices are known for both the reliability and the wide uses of their products. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. +44 118 944 8000. Nucleic Acid and Protein Quantitation. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. SpectraMax Microplate Readers. 303 unable to cal dark current. SoftMax Pro GxP Compliance Software. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. All test measurements and calculations are handlediD5. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. ATP (“flash” luminescence using . 2 in. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 3. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. 312 gain calibration failed. ZERO BIAS - scores, article reviews, protocol conditions and more. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Introduction. Description 1. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. 2 (Molecular Devices, San Jose, CA). Description 1. Find regional support. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. com. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. 1321N1, a human astrocytoma cell line. Myeloperoxidase (MPO) Assay. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Order Now. SpectraMax ® iD3 and iD5 Multi-Mode . 0´ 104 1. ATP (“flash” luminescence using . Price from $9. Cell viability was calculated as average percent of the OD reading relative to that of control group, which was normalized to 100%. Download PDF. 4 mg mL −1; 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. For wavelengths in the UV range above 220 nm,. SoftMax Pro. Increased fluorescence performance. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 0. 3. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. More Product Information. This instrument also provides the option of modular upgrades for Western Blotting,. 305 reference level saturation. Bioz Stars score: 98/100, based on 1 PubMed citations. 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Spectranet customer portalThe Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. china@moldev. Increased fluorescence performance. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. Each heating element is independently. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 9 x 10-15. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Also refer to this Multimode Reader Comparison Chart. 316 grating motor driver faulty. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. It allows you to explore cellular pathways and protein activation and expression in one system. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. Microplate Stacker. 1. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. User-upgradeable detection modules allow you to expand the capabilities of your microp. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The SoftMax Pro Software provides integrated instrument control. User-upgradeable detection modules allow you to expand the capabilities. 5 µL anti-cAMP-Cryptate. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Bioz Stars score: 98/100, based on 1 PubMed citations. 0. Get a front row view of your cells. 価格、見積もり、お近くの販売店を. Actual shake speed is based on the microplate format. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. Patented user-exchangeable cartridge design expands the system's detection capability making it. • Removing the transport locks from the PMT shutters and drawers. Microplate optimization should be performed the first time the assay is run, and subsequently for . The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. ; The top 3 importers of Spectramax are India with 365. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). 58% for the detection module and 2. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. pathways and protein activation and expression in one system. The chamber. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax i3x reader provides the. Multimode Readers Comparison Chart. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 01 0. The calculated LOQ values were 0. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Molecular Devices are known for both the reliability and the wide uses of their products. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. 315 can't find zero order. Table 1. Our patent-pending StainFree Cell Detection Algorithm enables cell counting and confluency measurements. In addition, Image J was used to quantify the. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. We would like to show you a description here but the site won’t allow us. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Bioz Stars score: 98/100, based on 1 PubMed citations. Learn more about its features, specifications, and applications in this user guide. The chamber. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Increased fluorescence performance. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology*On the SpectraMax i3x reader only. each new lot of plates, to ensure that the center of each well is read. 4. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. g. In dual reporter gene assays, cells are transfected with two vectors, the first containing an experimental reporter gene coupled to a regulated promoter of interest, andSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Register to download your free eBook today. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . 0. Find regional support. Hands-on fun and learning that empowers exploring, inventing and sharing. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 2. The calculated LOQ values were 0. However, no piece of equipment is immune to breaking down. QBT Fatty Acid Transporter Assay. *On the SpectraMax i3x reader only. Supplier: Molecular Devices. Example: [Drawer]. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Wokingham, Berkshire RG41 5TS United Kingdom. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. See SpectraMax® Microplate Reader User Manual Download Page. Donor emission at 616 nm is used as an internal reference, while acceptor emission at 665 nm. It allows you to explore cellular . Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. 99. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 吸収度マイクロプレートリーダー SpectraMax® Mini. The plate was covered and incubated for one hour at room temperature. To detect physical stability, Pdots were treated with water and incubated with plants. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA) using the setup of excitation and emission provided in the SoftMax Pro 7. 2. 315 can't find zero order. The treatments were. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Multimode Reader Comparison Chart. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. As. The corrected absorbance is within 5% of the. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 660-665 Eskdale Road. 10, and 0. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). 12911151) 0. 2 in. Introduction. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. To learn more about the device, please watch this short video. The SpectraMax ® i3x. See Unpacking the SpectraMax Paradigm Instrument on page 1. ) wide and 65 cm (25. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. 304 signal level saturation. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The information below applies to a standard 96 well plate, set to a linear shake mode. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Explore a wealth of applications with the future-ready SpectraMax i3x. 4 / 5. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. 635. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. The standard solutions (0. , 2011). 2 only) SpectraMax M-series Microplate readers. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. We would like to show you a description here but the site won’t allow us. Prime function 20 amol. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Explore a wealth of applications with the future-ready SpectraMax i3x. 모든 제품. 2. Introduction. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. This is not due to a temperature-dependent alteration in cellular autofluorescence,. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. The plate was shaken and incubated in the dark for 10 minutes. See SpectraMax® Microplate Reader User Manual Download Page. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Find regional support. ZERO BIAS - scores, article reviews, protocol conditions and more. Published Dec 16, 2020 | Updated Oct 10, 2023. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). 1. Home > Search Results > Danaher Inc > spectramax i3x. Find our technical support information along with contact details. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesThe SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. Get a front row view of your cells. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. 발광 (Luminescence) 리더기. Carbonyl assay The plates for carbonyl detection were prepared as described. Increased fluorescence performance. See SpectraMax® Microplate Reader User Manual Download Page. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Note: Additional readers with similar performance are listed. 1. 3) At the bottom of the strip, click on "Show. *On the SpectraMax i3x reader only. Prepare your i3/i3x instrument for shipment. Both Microplate Optimization and Read Height Adjustment were. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. VAL014, right table) assays on the SpectraMax i3x reader are shown below. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 7.